The United Church of Canada was formed in 1925, from a union of the Congregational Union of Canada, the Methodist Church and 70% of the Presbyterian Church in Canada, along with a number of churches organized under the General Council of Local Union Churches (mostly in Western Canada). Saskatchewan Conference was one of eleven regional Conferences established by the United Church of Canada General Council, at their inaugural meeting, in June 1925. Initially, the Conference contained 16 presbyteries but this number varied, in the years that followed.

In January 2019, as part of a larger restructuring of the United Church of Canada, Saskatchewan Conference and its seven existing presbyteries were combined into a single entity, initially identified as Region 4 and later renamed Living Skies Regional Council.