Regina Wascana Pastoral Charge was formed around 1932 (listed as vacant) but by 1936, the church was part of Regina Rosemont Pastoral Charge. Regina Wascana Pastoral Charge appears to have been re-formed as a joint charge with Rosemont, ca.1951, then as a separate charge, by 1953. Around 1970, preaching points were listed as Grand Coulee and Sherwood. Grand Coulee moved back into its own Pastoral Charge around 1973 but Sherwood remained until it closed, June 30, 1990. Wascana Pastoral Charge itself closed on September 30, 1996.

Regina Zion Pastoral Charge was formed as a new United Church charge in 1925, containing Zion United Church, in Regina. Around 1932, Zion was listed as part of Regina Westminster Pastoral Charge but the points later re-formed into separate charges. Regina Zion Pastoral Charge officially closed on June 30, 2015.

Regina St. Andrew's Pastoral Charge was formed as a new United Church charge in 1925, from what had previously been a Presbyterian field. On June 30, 2014, the church was closed and the congregation amalgamated with Knox-Metropolitan.

Regina Eastside Pastoral Charge was created as Prince of Peace Pastoral Charge (from the main preaching point), a new church development, on September 6, 1987. On January 1, 1998, the charge was renamed Eastside Pastoral Charge.

Regina Knox Pastoral Charge was formed as a new United Church charge in 1925, containing the preaching point Knox United Church, formerly Knox Presbyterian Church. The previous Presbyterian congregation dated back to around 1882. It built a church in 1885, at the corner of Scarth Street and 11th Avenue, and a later replacement in 1905.

In 1951, Knox United Church amalgamated with Metropolitan United Church, to become Knox-Metropolitan United Church (and Pastoral Charge), located on the site of the former Metropolitan United Church, at the corner of Victoria Avenue and Lorne Street.

Regina Westminster Pastoral Charge was formed as a new United Church charge in 1925, including Westminster United Church, itself an amalgamation of Westminster Presbyterian Church (at 3025 - 13th Avenue) and the nearby Fourteenth Avenue Methodist Church (in a temporary building on the southwest corner of 14th Avenue and Retallack Street). These congregations had originated from outreach efforts by the downtown Knox Presbyterian Church and Metropolitan Methodist Church and, in May 1925, they had combined their congregations, in the formerly Presbyterian building.

Regina Heritage Pastoral Charge was established January 1, 1984, from a preaching point previously part of Regina St. James Pastoral Charge.

Regina Presbytery was one of the original 16 presbyteries established by the first General Council of the United Church of Canada (June 1925), to be part of the new Saskatchewan Conference. Initial boundaries were based on the previous Regina Presbytery, which had been part of the Presbyterian Synod of Saskatchewan. Prior to Union, in 1925, there had also been a Regina District, in the Saskatchewan Conference of the Methodist Church.

In 2000, Saskatchewan Conference went from 10 presbyteries to 7, with each adopting a new name. Most pastoral charges from Regina Presbytery became part of the new Wascana Presbytery.